Authors:
Farah Fazwa, M.A.
; Nur Nazihah, M.
; Nihayah, M.
; Norhafizah, K.
; Syafiqah Nabilah, S.B.
; Norhayati, S.
; Mohd Zaki, A |
Abstract:
Labisia pumila var. alata (Kacip Fatimah) from Primulaceae family is a medicinal herb that
traditionally used by pregnant women especially before and after delivery process. Based on the
claimed, a research need to be carried out to study the function of L. pumila with uterine
contractility activity. Therefore this study is conducted with the main objective to select high
yielding accessions of L. pumila based on the contractility activity of uterus rats. A total of 30
accessions of L. pumila with high total phenolic content (TPC) selected from previous research
were used as research materials in this study. The rats were pretreated with diethylstilbestrol to
induce the estrus phase. The uterine were connected to the power lab apparatus. The 30
accessions of L. pumila extracts were injected into the organ bath with the concentration between
1.5 ug/ml to 3519.5 ug/ml, separately. As a results, four accessions were identified for having
higher contraction activity compared to the other accessions. The maximum force of contraction
were produced by LP15 (0.609+_0.126 g) followed by LP28 (0.593+_0.429g), LP26 (0.402+_0.15g)
and LP30 (0.276+_0.128 g) at different doses of concentrations. The four selected accessions were
then propagated using macropropagation through leaf cutting technique in order to evaluate the
propagation responses of the accessions. Among the variables measured were survival rate (%),
number of roots and root length (cm). From the observation, LP15 gave the highest survival rate
(87%) followed by LP28 (87%), LP30 (60%) and LP26 (36%). After six months of propagation
phase, rooted cuttings were transplanted and potted into 6" x 8" polybags containing (3 topsoil: 1
coco peat: 1 sand: 1 compost) potting medium and raised in nursery for another 12 weeks before
field planting. Variables such as leaf number, leaf width and leaf length were collected every month to evaluate the shoot production of the plants. As a result, LP15 recorded good growth in
terms of leaf number (4.6), whereas for leaf length, LP15 and LP26 recorded the highest value,
7.8 cm and 8.5 cm, respectively. Accession LP28 recorded the lowest value of leaf width (1.7
cm). The results from this study indicated that there was a relationship between function of L.
pumila with the uterine contractility activity. As a conclusion, these selected accessions will be
used as planting stocks for future breeding activities of the species. |
