Abstract:
A survey on the incidence of nepoviruses and vector nematodes were carried out in main grape
growing areas of Thrace Region, European part of Turkey. Grapevine Leafroll-Associated Virus
and Grapevine Fleck Virus were olso investigated in case of combination with any of
nepoviruses on grapevine. On this purpose randomly selected commercial vineyards were
sampled and total of 434 leaf samples were collected from grapevines exibiting virus like
symptoms to confirm the infection by Double antibody sandwich-enzyme linked immunosorbent
(DAS-ELISA) assay. Soil samples were also collected from rhizosphere of symptomatic
grapevines to evaluate the presence of vector nematode individuals. Six viruses including
Grapevine Leafroll-associated Virus 1 (GLRaV-1), Grapevine Leafroll-associated Virus 3
(GLRaV-3), Grapevine Fanleaf Virus (GFLV), Grapevine Fleck Virus (GFkV), Tobacco Black
Ringspot Virus (TBRV), Arabis Mosaic Virus (ArMV) were detected in vineyards. GFLV was
most predominant virus and out of 434 tested samples, 277 samples representing 57 % gave
positive reactions. Four virus vector nematodes Xiphinema index, Xiphinema diversicaudatum
Xiphinema italiae and Longidorus elongatus were identified in soils. |
