This study was carried out during the agricultural seasons 2016 - 2017 in three agricultural sites that were in the province of (Dhi Qar, Basra and Babylon) in order to estimate the most important chemical components of pollen of date palm cv semisemi and study of the pylogenetic effect of the ethanolic extract of the pollen by adopting of the random complete block design. Thirty-six trees of date palms were randomly selected from the group of trees planted in agricultural sites (Dhi Qar, Basrah and Babil) The bilogic experiment included the effect of concentrations (0, 50, 100, 200) mg / l of ethanolic extract of semisemi toxin pollen in inhibiting the growth diameter of Pathogenic fungi of humans, Candida albican and Aspergillus Niger. The most important results of the study were that the pollen of the Dhi Qar sit was significantly higher in dry matter (62.89%) and calcium, magnesium and boron (0.263, 1.380, 1.46) mg / g respectively. The lowest water content was achieved in the pollen of Thi-Qar Governorate (37.11%). Significant differences were found in the concentration of the amino acid proline and the high concentration was in the Samismi pollen of Basrah and Babil governorates (0.26,0.31)?gm/gm respectively. The results showed that the ethanolic extract of 200 mg / L inhibition of the growth rate of the fungus Candida albican was 15 mm higher than the other concentrations under study, while the highest inhibition of Aspergillus Niger was achieved with ethanol concentration 200, 100 mg / L and significantly different from the comparison treatment (distilled water).