Nineteen ISSR were used to investigate polymorphism among five Brassica species. ISSR
revealed 326 total bands the highest (89% polymorphism). 30 SRAP primer pairs revealed 480
bands with polymorphism (93%). ISSR and SRAP, both were effective in studying genetic
diversity in Brassica. Based on similarity matrix of overall analysis (ISSR and SRAP), the
dendrogram was constructed and separated the five Brassica into two main clusters. Results
showed that the length of the MYB28 gene for glucosinolates production at the fragment of 1257
bp. This information can be used in the future for breeding and improvement programs and helps
in national conservation programs.