IJAER

International Journal of Agriculture and Environmental Research™

ISSN 2455-6939

Title:
MOLECULAR CHARACTERIZATION OF SOME BRASSICA SPECIES

Authors:
R. M. A. Khalil, Mohamed Abd. S. El zayat

Abstract:
Nineteen ISSR were used to investigate polymorphism among five Brassica species. ISSR revealed 326 total bands the highest (89% polymorphism). 30 SRAP primer pairs revealed 480 bands with polymorphism (93%). ISSR and SRAP, both were effective in studying genetic diversity in Brassica. Based on similarity matrix of overall analysis (ISSR and SRAP), the dendrogram was constructed and separated the five Brassica into two main clusters. Results showed that the length of the MYB28 gene for glucosinolates production at the fragment of 1257 bp. This information can be used in the future for breeding and improvement programs and helps in national conservation programs.

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