Title: POLYAMINE ANALYSIS OF LEGUMINOUS SEED, SPROUT, LEAF,
FLOWER, POD, ROOT AND ROOT NODULE: DISTRIBUTION OF
DIAMINOHEXANE, AMINOBUTYLCADAVERINE,
METHYLPOLYAMINES AND ALKANOLPOLYAMINES |
Abstract: To determine organ-specific and species-specific polyamine distribution profiles in legumes
(leguminous plants), polyamines acid-extracted from a total 68 samples of the seeds, sprouts,
leaves, flowers, pods, roots and/or root nodules from 27 leguminous species belonging to the
family Fabaceae (formerly Leguminosae) were analyzed by high-performance liquid
chromatography and high-performance gas chromatography-mass spectrometry. Unusual tetraamines such as thermospermine, aminopropylhomospermidine, canavalmine and homospermine
were detected in the mature seeds but not in the immature seeds and other organs. An unusual
long aliphatic diamine, 1,6-diaminohexane, was found in the roots of ten species and the seeds of
two species. In the sprout (seedling), the root region increased in the levels of putrescine and
cadaverine, and the stem and seed parts increased in cadaverine level. 1,3-Diaminopropane,
norspermidine, norspermine and/or caldopentamine were distributed in some roots and leaves.
In addition to the high levels of cadaverine and homospermidine, both polyamines and
aminobutylcadaverine were found selectively in the root nodules of Phaseolus, Pueraria and
Vigna species. N
1
-Methylputrescine, N
1
,N
4
-dimethylputrecine, N
8
-methylspermidine, N
4
-
methylspermidine, N
1
-methylhomospermidine and/or N
5
-methylhomospermidine were
distributed within the root and root nodule of Glycine, Phaseolus, Pueraria and Vigna species. A
new N-methylated diamine, N
1
-methylcadaverine, was detected in the root of Canavalia gladiata
rich in cadaverine. New types of alkanolpolyamine (polyaminoalkylalcohol), N-(4-
aminobutyl)aminoethanol and/or N-(4-aminobutyl)aminopropanol, were found in the root
nodules of Glycine soja, Phaseolus vulgaris and in three Vigna species. Leguminous crop plants important for agriculture and foods are good sources for plant polyamine analyses.
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