IJAER

International Journal of Agriculture and Environmental Research™

ISSN 2455-6939

Title:
POLYAMINE ANALYSIS OF LEGUMINOUS SEED, SPROUT, LEAF, FLOWER, POD, ROOT AND ROOT NODULE: DISTRIBUTION OF DIAMINOHEXANE, AMINOBUTYLCADAVERINE, METHYLPOLYAMINES AND ALKANOLPOLYAMINES

Authors:
Koei Hamana, Takemitsu Furuchi, Hidenori Hayashi and Masaru Niitsu

Abstract:
To determine organ-specific and species-specific polyamine distribution profiles in legumes (leguminous plants), polyamines acid-extracted from a total 68 samples of the seeds, sprouts, leaves, flowers, pods, roots and/or root nodules from 27 leguminous species belonging to the family Fabaceae (formerly Leguminosae) were analyzed by high-performance liquid chromatography and high-performance gas chromatography-mass spectrometry. Unusual tetraamines such as thermospermine, aminopropylhomospermidine, canavalmine and homospermine were detected in the mature seeds but not in the immature seeds and other organs. An unusual long aliphatic diamine, 1,6-diaminohexane, was found in the roots of ten species and the seeds of two species. In the sprout (seedling), the root region increased in the levels of putrescine and cadaverine, and the stem and seed parts increased in cadaverine level. 1,3-Diaminopropane, norspermidine, norspermine and/or caldopentamine were distributed in some roots and leaves. In addition to the high levels of cadaverine and homospermidine, both polyamines and aminobutylcadaverine were found selectively in the root nodules of Phaseolus, Pueraria and Vigna species. N 1 -Methylputrescine, N 1 ,N 4 -dimethylputrecine, N 8 -methylspermidine, N 4 - methylspermidine, N 1 -methylhomospermidine and/or N 5 -methylhomospermidine were distributed within the root and root nodule of Glycine, Phaseolus, Pueraria and Vigna species. A new N-methylated diamine, N 1 -methylcadaverine, was detected in the root of Canavalia gladiata rich in cadaverine. New types of alkanolpolyamine (polyaminoalkylalcohol), N-(4- aminobutyl)aminoethanol and/or N-(4-aminobutyl)aminopropanol, were found in the root nodules of Glycine soja, Phaseolus vulgaris and in three Vigna species. Leguminous crop plants important for agriculture and foods are good sources for plant polyamine analyses.

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