Authors:
Mudita, I M., I G. Mahardika, I N. Sujaya, I. B. G. Partama |
Abstract:
A research has been carried out to evaluate the potency of bacteria isolated from bali cattle colon waste as lignocellulosic substrates degrader. Bacteria were isolated by Hungate selective media using combination of tannic acid (lignin), xylan, and Carboxy Methyl Cellulose/CMC (cellulose) as selective substrates. The potency of lignocellulolytic was identified based on the degradation of lignocellulosic substrates measurement using clear/diffusion zone width and lignocellulolytic enzyme activity. This study showed that lignocellulolytic bacteria isolated from bali cattle colon produce clear/diffusion zones in the width of wide 0.078 - 3.440 cm2, 0.007 - 0.072 cm2, 0.172 - 4.497 cm2 and 0,392 - 5,864 cm2 respectively for lignocellulose, tannic acid, cellulose and xylan substrates, while isolates coded BCC 7 LC, BCC 4 LC and BCC 12.1 LC produced higher clear/diffusion zones. Meanwhile for lignocellulolytic enzyme activity, bacteria isolates coded BCC 12.1 LC and BCC 6 LC has higher enzyme activity at tannic acid, CMC, and xylan substrates. It was concluded that bacteria isolates coded BCC 12.1 LC is a lignocellulosic substrates degrader. |
