Authors:
Esther Yap Shiau Ping, Siti Hajar Ahmad, Rosenani Abu Bakar, Khozirah Shaari, Surisa Phornvillay, Nur Indah Abdul Shukor, Tan Xue Yi
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Abstract:
Objective: To determine the effect of different types and rates of organic fertilizers on the
phytochemicals and chemical markers of O. stamineus.
Methods: Animal- and plant-based fertilizers with equivalent rates of 0, 100, 200, 300 and 400
kg N/ha were applied. Plants were then harvested at 8 weeks after transplanting for extraction
using 70% ethanol. Total phenolic contents (TPC) were determined by using the Folin-Ciocalteu
method, total flavonoid contents (TFC) by using complexation with aluminum chloride (AlCl3)
and antioxidant activity by DPPH radical scavenging assay. Sinensetin (SEN) and rosmarinic
acid (RA) were both quantified by high performance liquid chromatography (HPLC).
Results: TPC were not significantly affected by fertilizer types and rates. TFC showed a
quadratic regression trend for plant-based fertilizer. Both animal- and plant-based fertilizer
showed a linear decrease in DPPH as fertilizer rates increased. RA showed a linear decrease for
plant-based fertilizer as rates increased whereas a quadratic trend was seen for animal-based
fertilizer. SEN showed a quadratic trend only for animal-based fertilizer but no relationship for
plant-based fertilizer. |
