IJAER

International Journal of Agriculture and Environmental Research™

ISSN 2455-6939

Title:
INTERACTION BETWEEN CROPPING SYSTEMS AND STORAGE DURATION ON NITRATE, NITRITE, ANTIOXIDANT AND POSTHARVEST QUALITY OF Lactuca sativa

Authors:
Azimah, H; Ahmad, S. H.; Rosenani, A. B

Abstract:
Leafy green vegetables are highly demanded by Malaysian consumers due to their rich source of essential nutrients needed for good health. The increase usage of nitrogen fertilizers and livestock manure by growers to produce dark green leafy vegetables could lead to abundant chlorophyll pigment and a higher content of nitrate. The conversion of nitrate to nitrite and eventually to carcinogenic nitrosamines during storage could affect human health. Thus, a study was conducted to determine the effects of organic and conventional cropping systems and storage duration on the quality of leafy vegetables stored under refrigerated temperatures. Lactuca sativa were obtained from organic and conventional farms. Damaged-free vegetables were selected, cleaned, packed in perforated polyethylene bags and stored in a refrigerator at 6+_1 'C. Nitrate and nitrite contents, nitrate reductase (NR) activity, water loss, chlorophyll content, total phenolic content (TPC), total flavonoid content (TFC), 2, 2-diphenyl-2-picrylhydrazyl (DPPH), colour hue (h'), chromaticity (C*) and lightness (L*) were measured at 0, 3, 6 and nine days of storage. The experiment was conducted using a randomized complete block design, arranged in a factorial experiment, with five replications. The L. Sativa from the two cropping systems showed 4.5 to 8% weight loss and decreased firmness by 33 to 46% during storage. Both organic and conventional L. sativa showed a shift in colour from pale green to yellowish dull green due to the degradation of chlorophyll content. The organic and conventional L. sativa were 78% reduced in nitrate contents and 99% increase in nitrite content as storage days increased. The TPC and TFC both showed positive correlations with nitrate content while DPPH had the opposite. It can be suggested that L. sativa, should not be kept under refrigerated (6+_1 'C) storage for more than 3 days.

download full text