Abstract:
Rice husk abounds in many rice producing countries. Rice processing residues if not utilized
adequately can be a cause for environmental concern with regards to burning excesses. In this
study, the submerged fermentation of rice mill feed (RMF) by three indigenous fungi
(Aspergillus niger, Trichoderma viride and Rhizopus oryzae was demonstrated in pure and
mixed culture for the production of enzymes. Enzymes expressed were protease, phytase, ?amylase, glucoamylase, lignin peroxidase, pectinase and manganese peroxidase. Protease
activity was significantly (p<_0.05) high with A. niger (9.00+_1.00Iu/ml) than T. viride
(4.00+_0.58Iu/ml) and R. oryzae. (6.33+_0.33Iu/ml). phytase activity was numerically high in both
single and mixed culture submerged fermentation. Lignin peroxidase was significantly (p<_0.05)
higher in mixed culture of all three fungi (48.00+_2.31Iu/ml)
The fungi tested and enzymes synthesised have the potential for biodegradation of complex plant
material leading to modification, saccharification and nutrient release. This will in turn be
utilized beneficially in animal feed formulation and supplementation. Thereby making RMF a
valuable agro-industrial by-product from rice production.
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