EFFECT OF LEVEL OF PFD ON PHOTOSYNTHETIC PARAMETERS AND PRODUCTION OF STEVIOL GLYCOSIDES BY HYDROPONIC CULTURE FROM STEVIA REBAUDIANA BERTONI
Elsa Ventura Zapata, Gabriela Brito Uribe, Mirna Ma. Sánchez Rivera, Raúl A. Nava Juárez, Minerva Méndez Tinajero, Jonathán Sánchez Muñoz and Nadia P. Tapia Barrera
Elsa Ventura Zapata: Departamento de biotecnologia, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Código postal 62731, Yautepec, Morelos, México.
Gabriela Brito Uribe: Departamento de biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Código postal 62731, Yautepec, Morelos, México.
Mirna Ma. Sánchez Rivera: Departamento de interacción planta insecto, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Código postal 62731, Yautepec, Morelos, México.
Raúl A. Nava Juárez: Departamento de interacción planta insecto, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Código postal 62731, Yautepec, Morelos, México.
Minerva Méndez Tinajero: Departamento de biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Código postal 62731, Yautepec, Morelos, México.
Jonathán Sánchez Muñoz: Departamento de biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Código postal 62731, Yautepec, Morelos, México.
Nadia P. Tapia Barrera: Department of biotechnology Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional. Código postal 62731, Yautepec, Morelos, México.
ABSTRACT
Stevia rebaudiana produces steviol glycosides (GSt) 300 times sweeter higher than sugar cane. The problem is that the global demand is increasing and the agricultural production is insufficient. Is reported that Hydroponic cultivation and high irradiance increase the yield of S. rebaudiana and the production of steviol glycosides. This is due to the management and control of nutrition and the effect of light on the photosynthetic system, respectively. Therefore, the objective was to evaluate the effect on three levels of PFD (440.8, 383.9 and 273.2 μmol m-2s) efficiency of photosystem PSII, and production of glycosides of steviol in S. rebaudiana var. Morita II at 40 and 90 days, using a NFT hydroponic culture (Nutrient Film Technique). The highest photosynthetic rate was recorded at 440.8 μmol m-2s with 15.4 and 17.72 ppm CO2 min-1 g at 40 and 90 of culture days respectively and the higher content of steviol glycosides, with this same PFD, the lowest fluorescence emission occurred in PSII, while with low luminosity, the absorbed energy was harnessed more efficiently by the PSIIRC. The photosynthesis and production of steviol glycosides in S. rebaudiana were affected by level of PFD and culture time in a hydroponic system.
Keywords: NFT hydroponic system, Stevia rebaudiana, Irradiance, OJIP test, Chlorophyll (Chl) a fluorescence, CO2 assimilation