CHARACTERIZATION OF WHITE LUPIN (LUPINUS ALBUS) CULTIVARS’ RESPONSE TO PRIMARY INFECTION BY DIVERSE COLLETOTRICHUM LUPINI STRAINS FROM A SINGLE RUSSIAN FIELD
Samia Samad, Alina Baik, Stepan Boldyrev, Ivan Kotelevtsev, Polina Volkova, Ekaterina Bondarenko, Vladimir Bondarenko, Sofia Bitarishvili, Mikhail Lukashevich, Laurent Gentzbittel and Cécile Ben
Samia Samad: Skolkovo Institute of Science and Technology, Project Center for Agro Technologies, Bolshoy Boulevard 30, bld. 1, 121205, Moscow, Russia.
Alina Baik: Skolkovo Institute of Science and Technology, Project Center for Agro Technologies, Bolshoy Boulevard 30, bld. 1, 121205, Moscow, Russia.
Stepan Boldyrev: Skolkovo Institute of Science and Technology, Project Center for Agro Technologies, Bolshoy Boulevard 30, bld. 1, 121205, Moscow, Russia.
Ivan Kotelevtsev: Skolkovo Institute of Science and Technology, Project Center for Agro Technologies, Bolshoy Boulevard 30, bld. 1, 121205, Moscow, Russia.
Polina Volkova: Independent Researcher, Geel, Belgium.
Ekaterina Bondarenko: Department Molecular and cellular biology, Russian Institute of Radiology and Agroecology, 249032 Obninsk, Russia.
Vladimir Bondarenko: Department Molecular and cellular biology, Russian Institute of Radiology and Agroecology, 249032 Obninsk, Russia.
Sofia Bitarishvili: Department Molecular and cellular biology, Russian Institute of Radiology and Agroecology, 249032 Obninsk, Russia.
Mikhail Lukashevich: Russian Lupine Scientific Research Institute – branch of the Federal Williams Research Centre of Forage Production and Agroecology, 241524, Bryansk, Russia.
Laurent Gentzbittel: Skolkovo Institute of Science and Technology, Project Center for Agro Technologies, Bolshoy Boulevard 30, bld. 1, 121205, Moscow, Russia.
Cécile Ben: Skolkovo Institute of Science and Technology, Project Center for Agro Technologies, Bolshoy Boulevard 30, bld. 1, 121205, Moscow, Russia.
ABSTRACT
Anthracnose, induced by Colletotrichum lupini, significantly threatens the white lupin industry, primarily originating from infections in asymptomatic seeds. This study evaluated the aggressiveness of six C. lupini strains reisolated from symptomatic pods in the same field in 2021. The assessment targeted Russian cultivars (‘Aly Parus,’ ‘Michurinskij,’ ‘SN 5-19’) and the Australian cultivar ‘Andromeda.’
Employing a seed infection assay, seeds were directly inoculated with C. lupini mycelia, revealing morphological variations and distinct aggressiveness of the strains at different plant developmental stages. While none impeded plant emergence, four strains caused significant early mortality across all cultivars, with no discernible differences in susceptibility. This study briefly discusses utilizing survival analysis to comprehensively understand the fungus’s effects on plants. By exploring diverse tolerance strategies employed by white lupins during different developmental stages, it is suggested that the observed diversity in pathogenicity highlights the need for assembling diverse strains for effective screening and resistance breeding.
Keywords: Anthracnose, Legume, Pathological assays, Seed inoculation assay, Survival analysis