IN VITRO HOST-FREE CALLUS DEVELOPMENT AND PLANT REGENERATION OF OROBANCHE CRENATA FORSK. AN OBLIGATE ROOT-PARASITE
Mounia ENNAMI, Houda TAIMOURYA, Fatima zahra BRIACHE, Rabha ABDELWAHD, Lamiae GHAOUTI, Loubna BELQADI and Rachid MENTAG
Mounia ENNAMI: Biotechnology Unit, CRRA-Rabat, Avenue Mohamed Belarbi Alaoui, B.P: 6356, National Institute of Agricultural Research (INRA), Rabat, 10101, Morocco.
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Production, Protection and Plant Biotechnology Department, Institute of Agronomy and Veterinary Medicine Hassan II, Rabat, Morocco.
Houda TAIMOURYA: Production, Protection and Plant Biotechnology Department, Institute of Agronomy and Veterinary Medicine Hassan II, Rabat, Morocco.
Fatima zahra BRIACHE: Biotechnology Unit, CRRA-Rabat, Avenue Mohamed Belarbi Alaoui, B.P: 6356, National Institute of Agricultural Research (INRA), Rabat, 10101, Morocco.
Rabha ABDELWAHD: Biotechnology Unit, CRRA-Rabat, Avenue Mohamed Belarbi Alaoui, B.P: 6356, National Institute of Agricultural Research (INRA), Rabat, 10101, Morocco.
Lamiae GHAOUTI: Production, Protection and Plant Biotechnology Department, Institute of Agronomy and Veterinary Medicine Hassan II, Rabat, Morocco.
Loubna BELQADI: Production, Protection and Plant Biotechnology Department, Institute of Agronomy and Veterinary Medicine Hassan II, Rabat, Morocco.
Rachid MENTAG: Biotechnology Unit, CRRA-Rabat, Avenue Mohamed Belarbi Alaoui, B.P: 6356, National Institute of Agricultural Research (INRA), Rabat, 10101, Morocco.
ABSTRACT
This study investigates the development of an in vitro system for callus induction, root-like protrusion formation, and plant regeneration in Orobanche crenata, a parasitic plant of economic significance to legume crops. The research aims to establish a controlled environment for studying O. crenata biology and physiology independent of host plants, offering insights into its growth dynamics and potential applications in parasitic weed management. Various culture media and growth regulators were tested to optimize conditions conducive to callus initiation and subsequent differentiation. Results demonstrate that B5 medium supplemented with gibberellic acid (GA) at 20 mg L-1 effectively induces callus formation and supports the development of root-like structures, crucial for further studies on host-parasite interactions and potential therapeutic applications.
Keywords: Orobanche crenata, in vitro culture, callus induction, root-like protrusions, plant regeneration, parasitic plants, growth regulators